AFM Cryo

Morphology of a blend of two SBS block copolymers with different chain-architecture. AFM tapping mode, phase image, image size = 3 x 3 μm. Rameshwar Adhikari, Institut für Werkstoffwissenschaft, Martin-Luther-Universität, Halle-Wittenberg. Knife used: cryo AFM

Ultrastructure of the roundworm Caenorhabditis elegans. Thomas Müller-Reichert, EM Technology Development, MPI Dresden, and Kent McDonald, Electron Microscopy Laboratory, University of California, Berkley. Scale bar: 28 mm = 1 μm. Knife used: ultra 45

EM micrograph of an ultramicrotomed section of the anodic alumina fi lm formed on Al-2 wt%Cu alloy. Scale bar = 100 nm. Xiarong Zhou, School of Materials, University of Manchester. Knife used: ultra 35°

ultra sonic

Polycarbonate modified with rubber Jens Sicking, Bayer Technology Services, Leverkusen. Knife used: ultra sonic

peripheral nerve (rat)

Peripheral nerve (rat), HP frozen, freeze substituted, Epon embedded, cut with the ultra sonic knife, section thickness 50nm. W. Graber, Institut of Anatomy, University of Bern, Switzerland. Knife used: ultra sonic

Section obtained with the ultra sonic Maxi knife

Section obtained with the ultra sonic Maxi knife. Scale bar 1 µm. Knife used: ultra sonic Maxi


Serial section obtained with the ultra sonic Maxi knife at 55 nm from a high pressure frozen nematode sample. Knife used: ultra sonic Maxi

AFM amplitude image of the muscle of cat‘s mite Otodectes cynotis

AFM amplitude image of the muscle of cat‘s mite Otodectes cynotis. The contrast covers amplitude variation in the 1 – 3 nm range. Size of the whole image equals 4.6 microns. Nadejda Borisovna Matsko, Institut für angewandte Physik, ETH Zürich. Knife used: ultra AFM

Knife used: ultra 45° Jumbo

Knife used: ultra 45° Jumbo

Drosophila embryonic brain axons. Defocus -4µm, no phase plate Tomographic reconstruction, computational 5nm slice. M. Eltsov, IGBMC, Strasbourg Scale bar: 100nm. Knife used: CEMOVIS 25°

cryo immuno

a - g: Correlative super-resolution light and electron microscopy of Tokuyasu cryo sections from mouse kidney. a - b: Tom20 protein is expressed in outer membranes of mitochondria. c - f: pNCC is localized at plasma membranes and in microvilli. g: Similar results are obtained with immunogold on the expression of pNCC. Scale bars: a: 1 μm; c: 2 μm d: 1 μm; b, e, g: 0.5 μm Mateos et al., Sci. Rep. 6 34062 (2016) is licensed under CC BY 4.0. Knife used: cryo immuno