Perfect Loop for CryoSectioning
Step 1
Pull the sections away from the cutting edge of the diamond knife with an eyelash.
Step 2
Dip the LOOP in sucrose or sucrose/methyl-cellulose.
Step 3
Hold the LOOP above the cryo sections.
Step 4
Touch the sections with the almost frozen sucrose or sucrose methyl-cellulose. Gently lift up the LOOP and bring back to room-temperature.
Step 5
Lower the LOOP onto a grid and lift up again.
Step 6
Place the LOOP upside-down on a water surface to wash out the sucrose or sucrose methyl-cellulose and allow the grid to float free.
