Histo, Histo Cryo and Histo Jumbo Knives
The diamond knife for light microscopy
High quality diamonds guarantee
perfect sections and a durable edge
Easy wetting cutting edge
Optimized serial sectioning
Large boat for easy pick-up
histo
histo cryo
Rabbit joint, calcified cartilage/bone. X760
E. B. Hunziker, M. E. Müller Institute for Biomechanics,
University of Bern.View more images.
Area of application
The histo knife is designed for the sectioning of hard and soft biological and material research specimens, non embedded or embedded in acrylic or epoxy resins. (Ref. Reymond).
Our histo knives can be used on all ultramicrotomes as well as on microtomes with a retraction of the specimen arm in the return phase.
Our histo cryo knives are delivered with a boat for wet sectioning using a DMSO/water mixture or in triangular holders for dry sectioning. They are made from a special copper-nickel alloy, which guarantees
the best possible thermal conduction.
Resharpening service:
A re-sharpened Diatome diamond knife demonstrates the same high quality as a
new knife. Even knives purchased in previous years can continue to be resharpened.
histo Jumbo
For 3D reconstruction it is imperative not to lose a single section (Ref. Blumer).
The large Jumbo boat as well as the adhesive (Pattex compact by Henkel) applied to the side of the sample block increase the distinct advantages of our histo knives.
They allow:
- Easy production of section ribbons (0.5-2μm)
- No section loss
- No folding
- The same orientation of all sections
- Easy collection of section ribbons
- Multiple ribbons on one glass slide
- Perfect for Immuno-histo-chemistry
Eye of A. peroni: photographic sequence of
some semithin sections of a complete serie
through the eye.
L = lens; RE = retina.
Michael J.F. Blumer, Institut für Zoologie,
Universität Wien.
Reprinted from: Ribbons of semithin sections
an advanced method with a new type of
diamond knife. Journal of Neuroscience
Methods 120 (2002 11-16), with permission
from Elsevier. View more images.
References
O.L. Reymond: The «semi» diamond knife: a substitute for glass or conventional diamond knives in the ultramicrotomy of thin and semi-thin sections. Bas. Appl. Histochem. 30, pp. 487-494,
1986.
M.J.F. Blumer, P. Gahleitner, T. Narzt, C. Handl, B. Ruthensteiner: Strips of semi-thin sections: an advanced method with a new type of diamond knife. Journal of Neuroscience Methods 120,pp. 11-16,
2002.
M.J.F. Blumer: Development of a unique eye: photoreceptors of the pelagic predator Atlanta
peroni (Gastropoda, Heteropoda). Zoomorphology 119, pp. 81-91, 1996.
Ordering Information
All products can be ordered online using a credit card or purchase order number
for payment. Order online:
Diamond Knives and accessories can be ordered by phone at 215-412-8390 or fax 215-412-8450.