DiATOME Cryo Diamond Knives

A single slice of a tomogram of an aldehyde fixed and sucrose infiltrated cryosection with a 3D reconstruction. Erik Bos and Peter J. Peters, Netherlands Cancer Institute, Amsterdam. (see: J. Lefman, P. Zhang, T. Hirai, RM. Weis, J. Juliani, D. Bliss, M. Kessel, E. Bos, P.J. Peters, S. Subramaniam: Three-dimensional electron microscopic imaging of membrane invaginations in Echerichia coli overproducing the chemotaxis receptor Tsr. J. Bacteriol. 2004 Aug; 186(15): 5052-61.)

Fusion of late endosome/lyosome with the Mycobacteria tuberculosis phagosome. Aldehyde fixed cryo-section labeled with anti-CD63 and protein-A gold 10nm. Scale bar = 100nm. Nicole van der Wel and Peter J. Peters, Netherlands Cancer Institute, Amsterdam.

Ashraf Al-Amoudi, Laboratoire d'Analyse, Ultrastructurale, Lausanne.

High resolution electron micrograph of vitreous section of keratin intermediate filaments in the midportion of stratum corneum of human epidermis. The fine structure of the keratin filaments is well resolved and their molecular organization is seen in favorable cases (inset). Scale bar = 100nm. Scale bar inset = 20nm. < /br>From Ashraf Al-Amoudi, Laboratoire d'Analyse Ultrastructurale, Lausanne.

Cryosection, RuO4 stained. 11250x magnification. E. Ingolic, Technical University of Graz.

120,000x magnification. Ronald Walter, BASF Aktiengesellschaft, Polymer Physics, D-67056 Ludwigshafen.

25,000x magnification. Ronald Walter, BASF Aktiengesellschaft, Polymer Physics, D-67056 Ludwigshafen.

Immunogold labeling is present in the brushborder and endocytotic vesicles of a proximal tubular cell. 25,000x magnification. B. Guhl and J. Roth, Inst. for Cell- and Molecular Pathology, University of Zürich.

Cryosection. 55,000x magnification. M. Michel, M.E. Müller Inst. for Biomechanics, University of Bern.

129000x magnification. From E. Ingolic, Technical University of Graz.