DiATOME Cryo Diamond Knives

  • 3D Reconstruction
    A single slice of a tomogram of an aldehyde fixed and sucrose infiltrated cryosection with a 3D reconstruction. Erik Bos and Peter J. Peters, Netherlands Cancer Institute, Amsterdam. (see: J. Lefman, P. Zhang, T. Hirai, RM. Weis, J. Juliani, D. Bliss, M. Kessel, E. Bos, P.J. Peters, S. Subramaniam: Three-dimensional electron microscopic imaging of membrane invaginations in Echerichia coli overproducing the chemotaxis receptor Tsr. J. Bacteriol. 2004 Aug; 186(15): 5052-61.)
  • Fusion of late endosome/lyosome
    Fusion of late endosome/lyosome with the Mycobacteria tuberculosis phagosome. Aldehyde fixed cryo-section labeled with anti-CD63 and protein-A gold 10nm. Scale bar = 100nm. Nicole van der Wel and Peter J. Peters, Netherlands Cancer Institute, Amsterdam.
  • Desmosomes, Human Epidermis.
    Ashraf Al-Amoudi, Laboratoire d'Analyse, Ultrastructurale, Lausanne.
  • Vitreous section of keratin
    High resolution electron micrograph of vitreous section of keratin intermediate filaments in the midportion of stratum corneum of human epidermis. The fine structure of the keratin filaments is well resolved and their molecular organization is seen in favorable cases (inset). Scale bar = 100nm. Scale bar inset = 20nm. < /br>From Ashraf Al-Amoudi, Laboratoire d'Analyse Ultrastructurale, Lausanne.
  • Polypropylene/Polyamid blend, cryosection, RuO4 stained
    Cryosection, RuO4 stained. 11250x magnification. E. Ingolic, Technical University of Graz.
  • Polyethylene
    120,000x magnification. Ronald Walter, BASF Aktiengesellschaft, Polymer Physics, D-67056 Ludwigshafen.
  • Styrene-butadiene block copolymer
    25,000x magnification. Ronald Walter, BASF Aktiengesellschaft, Polymer Physics, D-67056 Ludwigshafen.
  • Localisation of megalin in an ultrathin cryosection of rat kidney
    Immunogold labeling is present in the brushborder and endocytotic vesicles of a proximal tubular cell. 25,000x magnification. B. Guhl and J. Roth, Inst. for Cell- and Molecular Pathology, University of Zürich.
  • High pressure frozen mature bovine articular cartilage tissue.
    Cryosection. 55,000x magnification. M. Michel, M.E. Müller Inst. for Biomechanics, University of Bern.
  • Polymethylpenten, cryosection, RuO4 stained.
    129000x magnification. From E. Ingolic, Technical University of Graz.
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